Microimaging Core
Paraffin embedding and sectioning
We can embed and cut your tissue using our TP1020 automated tissue processor, with fully customizable dehydration and infiltration sequences and times, to suit your tissue needs.
Whole-slide and targeted imaging
After cutting and staining, we can scan all your slides in their entirety, at high resolution (up to 40x objective) using a Zeiss slide scanner. Or, for more targeted imaging, we can take photos (transmitted or fluorescent) of your regions of interest.
Mouse lung H&E
Polarized light - human tendon, varying levels of collagen disorganization
human bone (tibia)
Mouse lung H&E
Equipment
Click on the names of the equipment from the list below for more information:
Leica CM3050 S Research Cryostat
Laser Microdissection Microscopes Leica LMD 7000
Internal Pricing VMRF/UCSD
Histology Technician & Handling Time (Total) 55$/Hr
Processing:
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Frozen Embedding 5$/Sample
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Frozen Sectioning 6$/Slide
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Paraffin Processing/Embedding 10$/Sample
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Paraffin Sectioning 3.5$/Slide
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H&E Staining 4$/Slide
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Special Staining, starting at 8$/Slide
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Immunostaining (with your provided antibody) 45$/Slide
Equipment Use:
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VevoMD Ultrasound 60$/Hr
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IVIS (In Vivo Imaging System) 50$/Hr
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Confocal Microscopy 43$/Hr
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Light Microscopy 10$/Hr
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Microdissection 30$/Hr
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Transmission Electron Microscopy 75$/Hr
Consultation/Training Time:
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Microscopy (Light/Confocal), IVIS, LMD, EM 100$/Hr
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Radiologist (VevoMD) 200$/Hr
Additional Material Fees:
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Slide Box, Small 5$
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Slide Box, Large 15$
Time billed in 15min increments, minimum 1hour..
External Pricing
Histology Technician & Handling Time (Total) 85$/Hr
Processing:
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Frozen Embedding 8$/Sample
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Frozen Sectioning 9$/Slide
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Paraffin Processing/Embedding 15$/Sample
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Paraffin Sectioning 6.5$/Slide
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H&E Staining 7$/Slide
-
Special Staining, starting at 11.5$/Slide
-
Immunostaining (with your provided antibody) 65$/Slide
Equipment Use:
-
VevoMD Ultrasound 100$/Hr
-
IVIS (In Vivo Imaging System) 72$/Hr
-
Confocal Microscopy 62$/Hr
-
Light Microscopy 15$/Hr
-
Microdissection 43$/Hr
-
Transmission Electron Microscopy 110$/Hr
Consultation/Training Time:
-
Microscopy (Light/Confocal), IVIS, LMD, EM 145$/Hr
-
Radiologist (VevoMD) 290$/Hr
Additional Material Fees:
-
Slide Box, Small 7.5$
-
Slide Box, Large 22$
Time billed in 15min increments, minimum 1hour..
Contact
The Microimaging Core Facility is located in the west wing of the 6th floor of the VA San Diego Healthcare Systems Hospital.
For information, to set up an order, or to set up a training appointment, please see contact box:
Jonathan Wong
Policies
Users are welcome to use choose to have our staff provide full service (see technician charge) or to coordinate a training session to use equipment themselves.
We ask that all users of this facility be respectful of other's schedules. Please arrive on time and conclude by transferring your images before the next person's appointment begins. If you know that you will be late for your appointment, please contact the Core Facility. Please remember that if you are late your time may be cut short if there is somebody scheduled directly after you.