Flow Cytometry Core
Overview
The Flow Cytometry Research Core facility provides a centralized resource for technical expertise and major equipment. The Core supports and enhances experimental design and execution of basic and applied research that require flow cytometric cell analysis or sorting.
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To achieve this objective, the Core offers the following services:
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Expert consultation is provided through the core director, laboratory supervisor, and our senior technical specialists;
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Cytometry instruments are selected for complementary functions;
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Equipment use is accessible through dedicated technician-operators;
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All services are provided on a recharge basis;
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Assistance with data analysis can be customized to the needs of individual investigators and research projects.
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The core facility contains five major pieces of equipment that perform multiple functions. Current services include: multiparameter analysis (1-6 antibody combinations) on cell suspensions and cells fixed to slides, and small to large volume cell sorting. Types of cell analyses include: cell maturation phenotypes; cell proliferation and activation (cell cycle/DNA content, CFSE dye; calcium flux, CD69, Annexin-V); intra-cellular proteins (cytokines, cell cycle regulators, viral products); quantification of molecular promoters and reporter genes (GFP, beta-gal; FRET).
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Live and fixed cell sorting is performed to recover cell subsets, identified with any combination of the above mentioned analysis tools. A specially equipped cell sorter is available for work with live, biohazardous cell samples (experimental protocol requires approval of the Core Director). Contact the facility to schedule services.