Metabolism Core
Overview
Provides excess human muscle and adipose tissue cultured cells for use of a tool to rapidly and effectively evaluate the biologic activity of new compounds in human muscle tissue using receptor reporting systems. Performs metabolic flux assays on cells.
Metabolism Core Costs
Cell culture: propagate hSMC from biopsy*
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Institutional Client: $1,760
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Outside Client: $2,492
Provide excess hSMC
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Institutional Client: $330
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Outside Client: $467
Maintain/treat adipose tissue or adipocytes in culture
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Institutional Client: $630/biopsy sample
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Outside Client: $892
Adipose tissue: isolate adipocytes
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Institutional Client: $250
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Outside Client: $354
Glucose uptake in adherent cells (2-4 plates)
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Institutional Client: $440
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Outside Client: $623
Determination of radioactivity in samples, correcting for counting efficiency and quenching
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Institutional Client: $3.30/sample (performed in duplicate)
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Outside Client: $4.40/sample (performed in duplicate)
FFA oxidation in adherent cells (2-4 plates)
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Institutional Client: $480
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Outside Client: $680
Glucose transport in suspended cells: cells either isolated by Metabolism Core or provided by client
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Institutional Client: $525
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Outside Client: $743
Pyrogenicity testing
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Institutional Client: $1,188
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Outside Client: $1,682
Assay: glucose specific activity in plasma
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Institutional Client: $423 (per assay)
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Outside Client: $599 (per assay)
Calculation of glucose production and utilization
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Institutional Client: $220
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Outside Client: $343
Multiplex analysis of cytokines (1 plate)
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Institutional Client: $170 or $400
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Outside Client: $241 or $566
Multiplex equipment operation
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Institutional Client: $110
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Outside Client: N/A
Long-term sample storage (-80ºC)
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Institutional Client: $5.50/box/month
Research data retrieval and collation
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Institutional Client: $29/hour
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Outside Client: $41/hour