MISSION

Those who courageously serve in our nation's military deserve exceptional care. The Veterans Medical Research Foundation's mission is to enhance the health of veterans of all generations through research and education.

GENERAL INFORMATION

​Phone:

(858) 642-3080

ADDRESS

3350 La Jolla Village Drive (151A)

Building 13

San Diego, CA 92161

Genomics and Sequencing Core

Overview

Recent advances in genome sequencing and high-throughput technology to analyze gene expression have placed biology at the forefront of modern science. The Genomics and Sequencing Core provides services, reagents and expertise to perform assays ranging from small-scale to large-scale analysis of gene expression and sequence data, microRNA detection, detection of SNPs and somatic mutations, TaqMan Low Density Arrays, and HIV and other viral quantitation. Projects related to HIV research are given additional assistance.

Equipment

The Genomics and Sequencing Core is equipped with the following instruments:

  • Agilent Bioanalyzer 2100

  • NanoDrop ND-1000

  • NanoDrop ND-2000c

  • Qiagen QIAcube

  • Techne TC-4000 & multiple other Thermal Cyclers

  • Applied Biosystems 7500 and 7900HT Real-time PCR systems (with multiple block types)

  • 2 Bio-Rad (formerly QuantaLife) Digital PCR instruments

  • Ion Torrent Personal Genome Machine & Ion Server

  • Illumina MiSeq Desktop Sequencer

Pricing

The Genomics and Sequencing Core services include:

  1. Validation of gene expression data and absolute quantitation of gene copy numbers using real-time quantitative qPCR technology (TaqMan and SYBRGreen) and droplet digital PCR (ddPCR).

  2. Ion Torrent PGM next generation sequencing assays, including viral genotyping, miRNA expression, and AmpliSeq™ and custom resequencing for detection of SNPs & somatic mutations.

  3. Bioinformatics analysis of gene expression and next generation sequencing data.

Sample preparation

Sample quantification

Gene expression quantification

SNP analysis

Bioinformatic analysis

Capillary population DNA sequencing

Viral quantitation assays

Viral qualitative assays

Viral and other genotyping assays

Lentiviral vector services

Ion Torrent next-generation sequencing

General pricing information

For more information about pricing for the Genomics and Sequencing Core, click the button below: