Download the Sample Submission Form. In order to comply with the MIAME standard (Minimum Information about a Microarray Experiment1), the core now requires users to fill out complete experimental and sample details.
RNA:
| Affymetrix Expression: |
3' IVT Expression- Starting amount 2.5-5 ug in 15 ul |
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Standard Ovation- Starting amount 200-500 ng in 10 ul |
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Whole Transcript Exon Chip- Starting amount 1.5 ug in 10 ul |
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Whole Transcript Gene 1.0 Chip- Starting amount 100 -200 ng in 10 ul |
| Nimblegen Expression: |
1-plex, 4-plex, or 12-plex- Starting amount 10 ug in 15 ul |
DNA: 454 Sequencing: The procedure requires 2-4 µg of double stranded DNA with a minimum concentration of 50ng/ul in TE Buffer. For LMW DNA, 70-800bp, 1microgram is required. The concentration should be verified by PicoGreen Fluorometry . Ideally, DNA starting material should be in pieces of >1.5Kb
SNP Assay: The Labelling Procedure requires 1µg of high quality genomic DNA with a minimum concentration of 100ng/ul.
Chromatin: A total of 4 µg of PCR amplified chromatin DNA in 8 µl volume (conc. of 500 ng/µl) may be submitted to perform ChIP-chip Hybridization Assays.
Samples should be submitted in person to the Core Laboratory along with the sample submission form, Monday through Friday from 10:00AM to 5:00 PM. It is best to schedule an appointment either by phone (858-552-8585-ext7100) or email (genechip@vapop.ucsd.edu) prior to submission of samples and chips. Samples may be placed in ice or dry ice (depending on distance traveled) and brought to the Core facility. All information including User, Sample, Chips and Service Request will be entered in the database upon arrival. Samples and Chips will then be numbered and stored at the appropriate temperature until lab work begins.
Chips: Chips may be bought from Affymetrix (1-888-DNA-CHIP) and shipped to the lab or directly to the Core.
Purchase Order Number: The sample submission form requires a Purchase Order Number (PO #) from the investigator’s institution for billing purposes.
1. Brazma et al. Minimum information about a microarray experiment (MIAME)—toward standards for microarray data, Nature Genetics, vol 29 (December 2001), pp 365 - 371. |
