Flow Cytometry Research Core

The Flow Cytometry Research Core has been established to provide a centralized resource of technical expertise and major equipment to support and enhance the experimental design and execution of basic and applied research that require flow cytometric analysis and/or cell sorting. To achieve our objective: expert consultation is provided through the core director and the laboratory supervisor; cytometry instruments are selected for complementary functions; equipment use is accessible through dedicated technician operators; services are provided on a recharge basis; and generated data are analyzed, reduced and diagramed per the requirements of individual investigators and their projects.

The core facility contains five major pieces of equipment that perform multiple functions. Current services include: multiparameter analysis (1-6 antibody combinations) on cell suspensions and cells fixed to slides, and small to large volume cell sorting. Types of cell analyses include: cell maturation phenotypes; cell proliferation and activation (cell cycle/DNA content, CFSE dye; calcium flux, CD69, Annexin-V); intra-cellular proteins (cytokines, cell cycle regulators, viral products); quantitation of molecular promoters and reporter genes (GFP, beta-gal; FRET).