Microimaging Core: Introduction


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The Microimaging Core Facility is located in the west wing of the basement of the VA San Diego Healthcare Systems Hospital. Approximately 1500 square feet are divided into a meeting/image processing room, a tissue preparatory room, one sectioning room with Reichert/Leica and RMC ultramicrotomes and Reichert/Leica microtome, one dedicated to a Zeiss EM10C electron microscope (with Gatan 785, 11 megapixel digital camera) and the other to a Zeiss LSM510 laser scanning confocal microscope, a Zeiss LSM5 Pascal laser scanning confocal microscope, and a Zeiss Light Microscope equipped with a digital camera. All microscope systems except the light microscope are connected to the UCSD network. Upstairs in the South wing of the 6th floor the Core houses a Leica Cryostat and a GE eXplore Micro CT.

The Microimaging Core Facility is managed on a daily basis by Director, Mark Murphy, M.D., Ph.D. who has 23 years experience in light and electron microscopy. Dr. Murphy is a full-time VA employee and an adjunct Associate Professor in the Department of Orthopedic Surgery, University of California, San Diego. As Director of the facility he oversees the day to day functioning of the Core Facility.

Jennifer Massie M.S. is the supervisor of the Microimaging Core, who has more than 35 years experience in histology. Jennifer is available through both e-mail (jmassie@ucsd.edu) and telephone (VA ext. 5939 or Cell: 858-335-6430) to answer any questions regarding tissue processing and the capabilities of the Microimaging Core Facility.

Michael Mashore is the lead technician at the Microimaging Core. He began studying Electron Microscopy while completing his Bachelor of Science in Biology at Humboldt State University. There he took multiple courses in both transmission electron microscopy and scanning electron microscopy. He worked at the Humboldt State Electron Microscopy lab for approximately 1 year before coming to the Microimaging Core.